Single-Cell Proteomic Pathway Omics

Identifying adaptive signaling pathways for designing improved targeted therapies

One of the biggest challenges in treating cancers is their ability to rapidly develop drug tolerance. Uncovering the mechanisms behind this cell-state change is vital but difficult to obtain with traditional methods. Researchers often look to genomic markers to understand the tumor microenvironment and signaling pathways; however, functional adaptations can occur in the tumor that are unrecorded in their genomic signatures.

Traditional flow-based methods detect a single cancer signaling pathway once it has been activated, so, once resistance develops and the tumor returns, additional experimental runs must be developed and performed to overcome this resistance. Using genomics alone has also led to challenges for researchers as providing a complete picture of a tumor’s mechanisms of therapeutic resistance is quite difficult and drugs targeted to those pathways have still resulted in drug tolerance.

Download the resource to learn how single-cell proteomic pathway omics can elucidate networked pathways, courtesy of Isoplexis.