The properties of the buffer used for ion exchange chromatography matter when it comes to isolating the right molecule
Ion exchange chromatography is used to separate molecules based on their total charge and is suitable for clinical, industrial, and drug development applications. Properties of a buffer such as pH, salts, detergent, and temperature will all affect whether the molecule of interest binds to the column resin. Optimizing these parameters will ensure that you are isolating the correct molecule every time.
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