Deconvolution is an image processing technique that reverses image degradation using the optical system’s point spread function
Light microscopes are diffraction limited, which means they are unable to resolve individual structures unless they are more than half the wavelength of light away from one another. With traditional widefield fluorescence
microscopy, out-of-focus light from areas above or below
the focal plane causes additional blurring in the captured
image.
Download the app note to learn more about:
- How deconvolution reconstructs an ideal image made from a collection of smaller point sources
- Deblurring, inverse filter, and constrained iterative algorithms
- Deconvolution in practice
Download the application note, courtesy of Olympus
