Newly Developed Global Plasma Proteome Quantification Workflow


Global plasma proteome quantification using internal standard triggered targeted analyses

The ability of plasma proteomics studies to deliver useful protein biomarkers has remained lower than initial expectations, prompting a redefinition of the plasma biomarker development pipeline. One proposed measure consisted in the implementation of a “rectangular” approach, relying exclusively on broad proteome profiling (through DDA or DIA analyses) across large cohorts for both the discovery and validation/verification stages. We describe here an alternative global quantification workflow, relying on a high density targeted acquisition method implemented on next generation Orbitrap mass spectrometers. This adaptation of the internal standard triggered PRM method leverages spiked-in stable isotopically labeled (SIL) peptides to drive the systematic screening of more than 500 plasma proteins per analysis across large sample sets.

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