Absolute quantification of target nucleic acids using partitioning
Digital polymerase chain reaction (dPCR) enables absolute quantification of target nucleic acids in a sample. The sample is partitioned into many individual reactions, each containing only a few target sequences. Following PCR amplification, amplified
target sequences are detected by fluorescence and the ratio of positive partitions to total partitions is used to determine the concentration of the target sequence in the sample. Quantification is based on the random distribution of molecules in multiple partitions that follows a Poisson distribution.
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