Untargeted metabolomics aims to comprehensively detect endogenous metabolites to generate a metabolic profile of a given biological system. This unbiased approach is often without a priori knowledge of the molecular make-up of a sample. Untargeted metabolite profiling globally provides the potential association of a metabolite or set of metabolites to a biochemical phenotype that can be further associated with metabolic pathways and biological function. These associations are insightful in an array of research and discovery settings such as defining signatures of disease and mechanisms of cellular function in human health, defining metabolic response in plant and animals resulting from a changing environment, or evaluating food composition in a commercial setting.
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