The Lab Manager PCR Resource Guide 

Authors: R. Ackerman; A. DePalma, PhD;  M. May, PhD; V. McLeod

PCR: What has changed and what hasn't?

PCR is a three-step, cyclic process, where new strands of DNA are created through successive rounds of denaturation, annealing, and extension using the enzyme DNA polymerase. 

PCR can fail for various reasons, in part due to its sensitivity to contamination causing amplification of spurious DNA products.

In this eBook you will learn:

  • Tips for successful preparation of the master mix
  • Advice in the event of no or low amplification
  • How to manage non-specific amplifications
  • Troubleshooting smeared bands

 

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